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Clean optical laboratory
Both optical systems, the Fluorescence microspetroscopy and Superresolution microscopy /nanoscopy are placed in the clean optical laboratory (ISO 6) together with modular platform for development of medical diagnostic technologies to ensure the best controllable and adaptable measurement environment at the same time. Small preparation lab is included as well to enable final modification of the samples before optical measurements. 

Tech details:  

• flow-based clean room of 48 square meters with HEPA air filtration (ISO 6 class) 
• 3 optic platforms (2PE STED mwFLIM, FMS+OT, modular platform for the development of photonics technologies) 
• standard lab technical lines (water, pressurized air, nitrogen, CO2, Ar) 
• sample storage and freezer 
• large set of optical components and tools 
• SoP for sample transfers and people flow 
• NFC access control 

Clean chemical and cell laboratory
Standardly equiped cell laboratory is placed in a clean room (ISO4/5) to ensure the most controllable environment to seed and grow cell lines. In addition, chemical clean laboratory (ISO5) is placed nearby to ensure the most efficient hadnling during preparation and basic characterization of the samples. Finally, on the end of the air flow of the clean rooms, there is nano laboratory (ISO 6) for handling and modification of nanomaterials as well as to clean the glass materials and sample holders.  

Tech details:  

• 3 flow-based clean rooms of total 58 m2 with HEPA air filtration (from ISO4/5 to ISO 6 class) 
• Standardly equiped clean cell laboratory (ISO4/5) with several incubators, laminar flow box, ultracentrifuge, deep freezer and small equipment 
• Standardly equiped clean chemical laboratory (ISO 5) with UV-VIS and FTIR spectrometers, centrifuges, pH-meters, mixers, shakers, fridges, small equipment, chemical boxes and extensive working surface 
• Nano clean laboratory (ISO 6) with two fume hoods, sonicators, rotational evaporators, autoclaves, washing machines and Vitrocell cloud aerosolization system 
• standard lab technical lines (water, pressurized air, nitrogen, CO2, Ar) 
• sample storage and freezers 
• extensive collection of spin and fluorescent probes 
• SoP for sample transfers and people flow 
• code access control 

Super-resolution microscopy / nanoscopy / nanospectroscopy
The Abberior GmbH state-of-the-art world first modular commercial 2-photon super-resolution 3D STED microscope equiped with several exciation and detection modalities for life ms imaging of supramolecular structures and function on the scales down to 30 nm with exceptionally high out-of-focus light rejection ability.  

Tech details:  

• Olympus body with several objectives, table, piezo controlled stage, LED widefield illumination  
• Abberior modular fully-software-controlled 3D STED system with  
• high-power Ti-Saph tunable wavelength 150 fs pulsed excitation laser source snychronized with  
• two fixed wavelengths 100 ps pulsed excitation laser sources and  
• fixed wavelength ns pulsed depletion laser  
• with 2 single-photon counters (avalanche photodiodes -APDs) as the main detectors with deadtime below 25 ns and  
• 16-channel PMT with diffraction grading and B&H FLIM module for spectrally-resolved FLIM detection 
• any crossection of the coordinates x,y,z,t,, possible with any of the combination of the following modalities: CF, 2D-3D STED, FLIM, spFLIM 
• flexible setup allows addition of new laser sources 
• flexible setup with incubation chamber for microscope-table 

Fluorescence microspectroscopy with optical tweezer and power broad-band light sources
The home-made modular spinning-disk-based confocal fluroescence microscope with the ability of spectral detection for fast spectrally-resolved imaging of environmentally sensitive probes, structures and interactions on the scales around 200 nm with spectral resolution of 1 nm and ability of NIR-tweezer-based manipulation. 

Tech details:  

• Nikon body with several objectives, table, piezo controlled stage, Xenon and several LED widefield illumination  
• spinning disk confocal unit 
• LCTF based spectral detection with Andor IXON EMCCED camera or 
• Rolera EMCCD camera 
• our own software control of all the units 
• including Aresis high-power NIR optical tweezer 

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Jožef Stefan Institute