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Tilen Koklic
Employment history 
2000 – present Researcher at "Jožef Stefan" Institute, Department of Solid State Physics, Laboratory of Biophysics, Ljubljana, Slovenia 
2013 – present Teaching Assistant: “Experimental Physics”, Faculty of Physics and Mathematics, University of Ljubljana, Ljubljana, Slovenia 
2011 – 2013 Teaching Assistant: “molecular and functional biology”, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia 
2010 – 2013 Researcher at Center of Excellence NAMASTE, Ljubljana, Slovenia 
Education  
 
Postdoctoral, Biochemistry and Biophysics,  
University of North Carolina at Chapel Hill, Chapel Hill, USA, 2005 – 2007 
• PEG mediated membrane fusion, supervisor: Barry R. Lentz 
• Factor Xa dimerization on PS-containing membranes, 
supervisor: Barry R. Lentz, and Rinku M. Majumder 
 
Doctor of Philosophy, Biophysics,  
University of Ljubljana, Ljubljana, Slovenia, 2000-2004 
• thesis title: Domain Structure Influence on the Interaction of Liposomes with Cells, supervisors: prof. dr. Milan Schara, and dr. Marjeta Šentjurc 
 
Bachelor of Science, Physics,  
University of Ljubljana, Ljubljana, Slovenia, 1995-2000 
• Thesis title: Domain Structure of liposome membranes made from alkylphospholipids and cholesterol as studiexd by EPR, supervisors: prof. dr. Milan Schara and dr. Marjeta Šentjurc 
 
Additional Education and working Experience, 
Visiting scientist at: 
• Reiner Zeisig's laboratory at Max Delbrück center for molecular medicine, Berlin-Buch, Germany, (2003 Nov - Dec) 
• Barry R. Lentz’s laboratory at University of North Carolina at Chapel Hill, Chapel Hill, USA, 2012 Apr, and 2013 April 29th to June 7th 
• Oliver Smithies laboratory at University of North Carolina at Chapel Hill, Chapel Hill, USA, 2013 April 29th to June 7thand august 2014 
Tilen Koklič organized media recognized visit of Nobel Prize winner for physiology or medicine for 2007 Oliver Smithies from the University of North Carolina at Chapel Hill School of Medicine. Prof. Smithies was visiting Jožef Stefan Institut from September 9th to 10th 2014, when he was greeted by prime minister of Slovenia and minister of Science. 
 
Exceptional Achievements & Research Experience, 
Tilen Koklič has more than 10 years of experience in the field of basic molecular mechanisms of blood coagulation gained during our collaboration with Barry R. Lentz’s laboratory from University of North Carolina at Chapel Hill and with prof Rinku Majumders’ laboratory from the Louisiana State University School of Medicine in New Orleans, both recognized experts in the field of blood coagulation. Tilen Koklič showed, as the first author, heretofore yet unknown molecular mechanisms regarding regulation of factor Xa and of the prothrombinase (fXa-fVa) complex (Biochemical Journal, IF 4.40, Biophysical Journal, IF 4,32; Biochemistry, IF 3.56).  
 
1. Koklic, T., Majumder, R. & Lentz, B. R. Ca2+ switches the effect of PS-containing membranes on Factor Xa from activating to inhibiting: implications for initiation of blood coagulation. Biochem. J. 462, 591–601 (2014). 
2. Koklic, T., Chattopadhyay, R., Majumder, R. & Lentz, B. R. Factor Xa dimerization competes with prothrombinase complex formation on platelet-like membrane surfaces. Biochem. J. 467, 37–46 (2015). 
3. Koklic, T., Majumder, R., Weinreb, G. E. & Lentz, B. R. Factor XA binding to phosphatidylserine-containing membranes produces an inactive membrane-bound dimer. Biophys. J. 97, 2232–2241 (2009). 
4. Majumder, R., Koklic, T., Rezaie, A. R. & Lentz, B. R. Phosphatidylserine-induced factor Xa dimerization and binding to factor Va are competing processes in solution. Biochemistry 52, 143–151 (2013). 
 
He was the principal investigator of the project financially supported by the Slovenian Research Agency ARRS Project J3-6803, ”Role of Calcium and lipid membranes in survival of critically ill patients”, and ARRS project BI-US/13-14-040 ”Physiological role of factor Xa and protein S in coagulation and inflammation processes”, where he collaborated with the Nobel Prize winner of physiology or medicine for 2007 Oliver Smithies from the University of North Carolina at Chapel Hill School of Medicine. Tilen Koklič published with him a joint publication, as a corresponding author, in journal Langmuir (IF 4.4).  
 
1. Smithies, O. et al. & Koklic, T.* Stable oligomeric clusters of gold nanoparticles: preparation, size distribution, derivatization, and physical and biological properties. Langmuir 30, 13394–13404 (2014). 
2. Lawrence, M., Testen, A., Koklic, T. & Smithies, O. A Simple Method for the Size Controlled Synthesis of Stable Oligomeric Clusters of Gold Nanoparticles under Ambient Conditions. J Vis Exp e53388 (2016). 
 
He has over 10 years of experience in the field of nanomaterial synthesis, characterization, and interaction of nanomaterials with living cells and cellular barriers (ACS Nano Letters IF 12.8, A'', Small, IF 9.59, A’’; Biomedical Optics Express, IF 2.33; PLOS One IF 3.03),  
 
1. Urbančič, I. et al. including Koklic, T. Nanoparticles Can Wrap Epithelial Cell Membranes and Relocate Them Across the Epithelial Cell Layer. Nano Lett. 18, 5294–5305 (2018). 
2. Aničić, N., Vukomanović, M., Koklic, T. & Suvorov, D. Fewer Defects in the Surface Slows the Hydrolysis Rate, Decreases the ROS Generation Potential, and Improves the Non-ROS Antimicrobial Activity of MgO. Small 14, (2018). 
3. Arsov, Z. et al. including Koklic, T. Fluorescence microspectroscopy as a tool to study mechanism of nanoparticles delivery into living cancer cells. Biomed Opt Express 2, 2083–2095 (2011). 
4. Garvas, M. et al. including Koklic, T.* Protein Corona Prevents TiO2 Phototoxicity. PLoS ONE 10, e0129577 (2015). 
 
 
 
and about 15 years of experience with culturing of cells and epithelial barriers (BBA Biomembranes, IF 4.18; Chemistry and Physics of Lipids, IF 2.42; Journal of Pharmaceutical Sciences, IF 3.03). 
 
1. Koklic, T., Zeisig, R. & Sentjurc, M. Interaction of alkylphospholipid liposomes with MT-3 breast-cancer cells depends critically on cholesterol concentration. Biochim. Biophys. Acta 1778, 2682–2689 (2008). 
2. Koklic, T. Perifosine induced release of contents of trans cell-barrier transport efficient liposomes. Chem. Phys. Lipids 183, 50–59 (2014). 
3. Orthmann, A. et al. including Koklic, T. Impact of membrane properties on uptake and transcytosis of colloidal nanocarriers across an epithelial cell barrier model. J Pharm Sci 99, 2423–2433 (2010). 
 
 
Grants, Awards, Distinctions and Fellowships  
• 2014-2017 principal investigator in ARRS Project J3-6803, 'Role of Calcium and lipid membranes in survival of critically ill patients' 
• 2013-2014 principal investigator in ARRS project BI-US/13-14-040 'Physiological role of factor Xa and protein S in coagulation and inflammation processes' 
• 2013 joined first author paper was selected as highlighted article in Biochemisty journal 
• 2000-2004 Young Researcher Scholarship 
• 1992-2000 Zois Scholarship for talented Students 
• 1993 Award for physics research project "Waves on Water Surface" 
• 1992 third prize at the national high school physics competition 
• 1990 Golden award of Jurij Vega at the national high school competition 
 
Selected Invited Reasearch Talks: 
• Regional Biophysics Conference (RBC), Kladovo, Serbia, 3-7.09.2012 
• Regional Biophysics Conference (RBC), Primošten, Croatia, 15-18.09.2010 
• conference contribution selected for oral presentation, 51st ASH Annual Meeting and Exposition, December 5-8, 2009, New Orleans, LA, USA 
(app. 10 % of contributions are usually selected for oral presentation) 
Mentorships 
• Co-mentor at the Master's Degree (Bologna Studies) of Anže Testen: 
TESTEN, Anze. Influence of albumin on phototoxicity of TiO2 nanotubes: master's thesis: master's study, 2014.  
Anže Testen defended the master's thesis with the highest grade and was accepted as a doctoral student, among approximately 1,400 applicants, at the University of North Carolina, USA, as one of seven foreign students. 
• Co-mentor at the diploma thesis of Rok Podlipec: 
PODLIPEC, Rok. Study of the transport of alkylphospholipids through the membrane of malignant cells with electronic paramagnetic resonance: thesis. Ljubljana: [R. Podlipec], 2011. 
Rok Podlipec advocated a master's thesis with the highest grade. 
 
Community Involvement / Administrative Activity  
Helped in writing proposals for: 
• Target Research Programme of doc. dr. Janez Štrancar: V4-0522 (D) – Antimicrobial nanocoatings for safe food processing (Protimikrobni nanonanosi pri varni predelavi hrane),  
• Basic Research Programme of dr. Marjeta Šentjurc: J7-0337 (D) – Design and characterization of fuzogenic coloidal nanoparticles for efficient transport of drugs into cells (Izdelava in karakterizacija fuzogenih koloidnih nanodelcev za učinkovit prenos učinkovin v celice), 
• Research Programme of doc. dr. Janez Štrancar: P1―0060 - Experimental biophyics of complex systems (Eksperimentalna biofizika kompleksnih sistemov), 
• Center of Excellence of prof. dr. Marija Kosec: Namaste 2010. 
• Pre-proposal for MNT-ERA.NET 2010 Transnational Call of doc. dr. Janez Štrancar: Targeting AntimicroBial Activity via micro/Nano-structured surfaces for civil Applications 

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