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Tilen Koklič
researcher in 
Laboratory of Biophysics, Condensed Matter Physics Department, Jožef Stefan Institute
Jamova cesta 39, SI-1000 Ljubljana, Slovenia

Contact:

tilen.koklic@ijs.si | +386 1 4773 438 | Room J110 | Twitter | Google Scholar | ReserchGate | ORCID



Education:

2005–2007       Postdoc in biophysics and biochemistry, University of North Carolina at Chapel Hill, USA
2000–2004       PhD in biophysics, University of Ljubljana, Slovenia
2009–2004       Diploma in physics, University of Ljubljana, Slovenia


Employment:

2000–present   Researcher in Laboratory of Biophysics, Jožef Stefan Institute, Ljubljana, Slovenia
2011–present   Teaching assistant, University of Ljubljana, Ljubljana, Slovenia
2010–2013       Researcher at Center of Excellence NAMASTE, Ljubljana, Slovenia

 
Research fields:
  • molecular mechanisms of blood coagulation
  • synthesis, characterization, and interaction of nanomaterials with living cells and cellular barriers
 
Additional education and working experience:
Visiting scientist at: 
  • Reiner Zeisig's lab at Max Delbrück center for molecular medicine, Berlin-Buch, Germany (2003)
  • Barry R. Lentz’s laboratory at University of North Carolina at Chapel Hill, USA (2012, 2013)
  • Oliver Smithies laboratory at University of North Carolina at Chapel Hill, USA (2013, 2014)

Exceptional achievements & research experience:

Tilen Koklič has more than 10 years of experience in the field of basic molecular mechanisms of blood coagulation gained during our collaboration with Barry R. Lentz’s laboratory from University of North Carolina at Chapel Hill and with prof Rinku Majumders’ laboratory from the Louisiana State University School of Medicine in New Orleans, both recognized experts in the field of blood coagulation. Tilen Koklič showed, as the first author, heretofore yet unknown molecular mechanisms regarding regulation of factor Xa and of the prothrombinase (fXa-fVa) complex (Biochemical Journal, IF 4.40, Biophysical Journal, IF 4,32; Biochemistry, IF 3.56).  
  1. Koklic, T., Majumder, R. & Lentz, B. R. Ca2+ switches the effect of PS-containing membranes on Factor Xa from activating to inhibiting: implications for initiation of blood coagulation. Biochem. J. 462, 591–601 (2014). 
  2. Koklic, T., Chattopadhyay, R., Majumder, R. & Lentz, B. R. Factor Xa dimerization competes with prothrombinase complex formation on platelet-like membrane surfaces. Biochem. J. 467, 37–46 (2015). 
  3. Koklic, T., Majumder, R., Weinreb, G. E. & Lentz, B. R. Factor XA binding to phosphatidylserine-containing membranes produces an inactive membrane-bound dimer. Biophys. J. 97, 2232–2241 (2009). 
  4. Majumder, R., Koklic, T., Rezaie, A. R. & Lentz, B. R. Phosphatidylserine-induced factor Xa dimerization and binding to factor Va are competing processes in solution. Biochemistry 52, 143–151 (2013). 
 
He was the principal investigator of the project financially supported by the Slovenian Research Agency ARRS Project J3-6803, ”Role of Calcium and lipid membranes in survival of critically ill patients”, and ARRS project BI-US/13-14-040 ”Physiological role of factor Xa and protein S in coagulation and inflammation processes”, where he collaborated with the Nobel Prize winner of physiology or medicine for 2007 Oliver Smithies from the University of North Carolina at Chapel Hill School of Medicine. Tilen Koklič published with him a joint publication, as a corresponding author, in journal Langmuir (IF 4.4).   
  1. Smithies, O. et al. & Koklic, T.* Stable oligomeric clusters of gold nanoparticles: preparation, size distribution, derivatization, and physical and biological properties. Langmuir 30, 13394–13404 (2014). 
  2. Lawrence, M., Testen, A., Koklic, T. & Smithies, O. A Simple Method for the Size Controlled Synthesis of Stable Oligomeric Clusters of Gold Nanoparticles under Ambient Conditions. J Vis Exp e53388 (2016). 
 
He has over 10 years of experience in the field of nanomaterial synthesis, characterization, and interaction of nanomaterials with living cells and cellular barriers (ACS Nano Letters IF 12.8, A'', Small, IF 9.59, A’’; Biomedical Optics Express, IF 2.33; PLOS One IF 3.03),  
  1. Urbančič, I. et al. including Koklic, T. Nanoparticles Can Wrap Epithelial Cell Membranes and Relocate Them Across the Epithelial Cell Layer. Nano Lett. 18, 5294–5305 (2018). 
  2. Aničić, N., Vukomanović, M., Koklic, T. & Suvorov, D. Fewer Defects in the Surface Slows the Hydrolysis Rate, Decreases the ROS Generation Potential, and Improves the Non-ROS Antimicrobial Activity of MgO. Small 14, (2018). 
  3. Arsov, Z. et al. including Koklic, T. Fluorescence microspectroscopy as a tool to study mechanism of nanoparticles delivery into living cancer cells. Biomed Opt Express 2, 2083–2095 (2011). 
  4. Garvas, M. et al. including Koklic, T.* Protein Corona Prevents TiO2 Phototoxicity. PLoS ONE 10, e0129577 (2015). 
   
and about 15 years of experience with culturing of cells and epithelial barriers (BBA Biomembranes, IF 4.18; Chemistry and Physics of Lipids, IF 2.42; Journal of Pharmaceutical Sciences, IF 3.03). 
  1. Koklic, T., Zeisig, R. & Sentjurc, M. Interaction of alkylphospholipid liposomes with MT-3 breast-cancer cells depends critically on cholesterol concentration. Biochim. Biophys. Acta 1778, 2682–2689 (2008). 
  2. Koklic, T. Perifosine induced release of contents of trans cell-barrier transport efficient liposomes. Chem. Phys. Lipids 183, 50–59 (2014). 
  3. Orthmann, A. et al. including Koklic, T. Impact of membrane properties on uptake and transcytosis of colloidal nanocarriers across an epithelial cell barrier model. J Pharm Sci 99, 2423–2433 (2010). 
  

Grants, awards, distinctions and fellowships: 
2014-2017 - principal investigator in ARRS Project J3-6803, 'Role of Calcium and lipid membranes in survival of critically ill patients' 
2013-2014 - principal investigator in ARRS project BI-US/13-14-040 'Physiological role of factor Xa and protein S in coagulation and inflammation processes' 
2013             joined first author paper was selected as highlighted article in Biochemisty journal 
2000-2004 - Young Researcher Scholarship 
1992-2000 - Zois Scholarship for talented Students 
1993 -          Award for physics research project "Waves on Water Surface" 
1992 -          third prize at the national high school physics competition 
1990 -          Golden award of Jurij Vega at the national high school competition 
 

Selected invited reasearch talks: 
2012 - Regional Biophysics Conference (RBC), Kladovo, Serbia
2010 - Regional Biophysics Conference (RBC), Primošten, Croatia  
2009 - contribution selected for oral presentation, 51st ASH Annual Meeting and Exposition, New Orleans, LA, USA (app. 10 % of contributions are usually selected for oral presentation) 


Mentorships: 
  • Co-mentor at the Master's Degree (Bologna Studies) of Anže Testen
    TESTEN, Anze. Influence of albumin on phototoxicity of TiO2 nanotubes: master's thesis: master's study, 2014.
    Anže Testen defended the master's thesis with the highest grade and was accepted as a doctoral student, among approximately 1,400 applicants, at the University of North Carolina, USA, as one of seven foreign students. 
  • Co-mentor at the diploma thesis of Rok Podlipec: 
    PODLIPEC, Rok. Study of the transport of alkylphospholipids through the membrane of malignant cells with electronic paramagnetic resonance: thesis. Ljubljana: [R. Podlipec], 2011. 
    Rok Podlipec advocated a master's thesis with the highest grade. 

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